| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.35[luc2P/9XGAL4UAS/Hygro] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 6184 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pGL4.35[luc2P-9XGAL4UAS-Hygro]载体图谱](/uploads/载体图谱/pGL4.35[luc2P-9XGAL4UAS-Hygro]载体图谱.png)
载体简介
载体序列
LOCUS pGL4.35[luc2P 9XGAL4UAS Hygro] 6184 bp ds-DNA circular SYN 27-JUL-2013
DEFINITION Vector with a promoter and an extended Gal4 upstream activating
sequence for studying cell signaling using destabilized luciferase.
ACCESSION GQ229577
VERSION .
KEYWORDS pGL4.35[luc2P 9XGAL4UAS Hygro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6184)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Wednesday, February 19, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6184
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
protein_bind 31..212
/bound_moiety="Gal4"
/note="9X GAL4 UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 222..262
/note="Ad promoter"
/note="adenovirus major late promoter"
CDS 315..1964
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 1968..2087
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 2139..2260
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2454..2811
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2662..2797
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2842..3879
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
polyA_signal 3903..3951
/note="synthetic polyadenylation signal"
rep_origin complement(4278..4866)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5066..5926)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 6031..6079
/note="synthetic polyadenylation signal"
misc_feature 6093..6184
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
61 cctccgactc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
121 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
181 ctccgaggaa ttccggagta ctgtcctccg aagacgctag cggggggcta taaaaggggg
241 tgggggcgtt cgtcctcact ctagatctgc gatctaagta agcttggcaa tccggtactg
301 ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
361 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
421 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
481 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
541 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
601 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
661 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
721 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
781 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
841 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
901 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
961 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
1021 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
1081 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
1141 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
1201 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
1261 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
1321 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
1381 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
1441 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
1501 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
1561 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
1621 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
1681 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
1741 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
1801 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
1861 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
1921 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgaattct cacggcttcc
1981 ctcccgaggt ggaggagcag gccgccggca ccctgcccat gagctgcgcc caggagagcg
2041 gcatggatag acaccctgct gcttgcgcca gcgccaggat caacgtctaa ggccgcgact
2101 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
2161 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
2221 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
2281 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
2341 caaatgtggt aaaatcgata aggatccgtt tgcgtattgg gcgctcttcc gctgatctgc
2401 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttagctac cttctgaggc
2461 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
2521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
2581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
2641 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
2701 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
2761 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgat
2821 tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag cgttgaaaaa
2881 tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga gggcgaagag
2941 agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt gaatagctgc
3001 gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc tgcactaccc
3061 atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg catcagtaga
3121 cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc tgtgttacag
3181 cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac cagcggcttc
3241 ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt catttgcgcc
3301 attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt gtccgccagc
3361 gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga ggtgcgccac
3421 ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg catcaccgcc
3481 gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc caacatcttc
3541 ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga gcgccggcat
3601 cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg cctggatcag
3661 ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca aggccgctgc
3721 gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc tcgccggagc
3781 gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa ccgccggccc
3841 agtacacgac cgcgcgctaa ggaggtaggt cgagtttaaa ctctagaacc ggtcatggcc
3901 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta
3961 gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
4021 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
4081 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
4141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
4681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
4921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
4981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
5041 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
5101 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
5161 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
5221 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
5281 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
5341 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
5401 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
5461 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
5521 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
5581 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
5641 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
5701 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
5761 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
5821 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
5881 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
5941 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
6001 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
6061 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
6121 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
6181 ctct
//
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